Palestrante: Dr. Ernesto S. Nakayasu
Biological Sciences Division, Pacific Northwest National Laboratory, Richland, WA, USA
Post-translational modifications (PTMs) are important regulators of the activity, localization and degradation of proteins in cells. Often, PTMs are part of complex signaling networks and how they regulate the signal transduction is not well understood. We have been developing and applying mass spectrometry-based systems approaches to study the function of PTMs in bacterial physiology and virulence. We have performed a phyloproteomics analysis to identify common PTMs across the bacterial evolution. With such approach we have been discovering conserved mechanisms of protein regulation. We have also been studying the function of PTMs in bacterial virulence, with a special focus in ubiquitination. We have characterized novel mechanisms of ubiquitination by bacterial virulence factors, which had no precedents in the literature. Overall, our systems approach has been shown to be effective in determine the role of PTMs.